Product Datasheet  
RAD9A Monoclonal Antibody  
Catalog Number: 27193  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Mouse
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Uniprot: Q99638

    Gene ID: 5883

  • Form of Antibody：  
  • Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
  • Storage：  
  • store at -20˚C
  • Immunogen：  
  • Purified recombinant human Rad9A protein fragments expressed in E.coli
  • reactivity：  
  • Hu Ms Rt Mk
  • appl_detail：  

  •  Western blotting: 1:500

  • other_names：  
  • Cell cycle checkpoint control protein; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A;
  • Purification：  
  • Affinity purified
  • Specificity：  
  • This antibody detects endogenous levels of RAD9A,and does not cross-react with related proteins.
  • Applications：  
  • WB IP
  • Background：  

  •  Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3'->5' double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.




 
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