Product Datasheet  
DNA-PKcs Monoclonal Antibody  
Catalog Number: 27182  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Mouse
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Uniprot: P78527

    Gene ID: 5591

  • Form of Antibody：  
  • Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
  • Storage：  
  • store at -20˚C
  • Immunogen：  
  • Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:1000

    Immunocytochemistry: 1:200
  • other_names：  
  • DNA dependent protein kinase catalytic subuni; DNA PKcs; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNAPK; DNPK1; hyper radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; p350; p460; PRKDC;
  • Purification：  
  • Affinity purified
  • Specificity：  
  • This antibody detects endogenous levels of DNA-PKcs,and does not cross-react with related proteins.
  • Applications：  
  • WB IP ICC
  • Background：  

  •  properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.Required to protect and align broken ends of DNA.May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.Found at the ends of chromosomes,suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion.Also involved in modulation of transcription.Recognizes the substrate consensus sequence[ST]-Q.Phosphorylates'Ser-139' of histone variant H2AX/H2AFX,thereby regulating DNA damage response mechanism.Phosphorylates DCLRE1C,c-Abl/ABL1,histone H1,HSPCA,c-jun/JUN,p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2.Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA.Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.




 
© Signalway Biotechnology All Rights Reserved.