Product Datasheet  
Bcr(Phospho-Tyr177) Antibody  
Catalog Number: 15199  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P11274
    NCBI Protein: NP_004318.3
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 177 (P-F-Y(p)-V-N) derived from Human Bcr.
  • reactivity：  
  • Hu Ms
  • appl_detail：  
  • Predicted MW: 210kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • BCR; BCR protein; BCR1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177.
  • Applications：  
  • WB IHC
  • Background：  
  • GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

    Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
    Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
    Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
    Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907



 
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