GST Polyclonal Antibody

Catalog Number:

27221

Amount:

100μg

Price:

$319

Swiss-Prot No:

Uniprot:

Gene ID: 8113787

Form of Antibody:

Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purified recombinant Escherichia coli BL21(DE3) GST protein fragments expressed in E.coli.

Purification:

Affinity purified

Specificity/Sensitivity:

Transfected Only

Applications:

WB

Background:

The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial,and microsomal (now designated as MAPEG) proteins.GSTs are present in eukaryotes and in prokaryotes,where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates.Members of the GST superfamily are extremely diverse in amino acid sequence,and a large fraction of the sequences deposited in public databases are of unknown function.The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.GSTs can constitute up to 10% of cytosolic protein in some mammalian organs.GSTs catalyse the conjugation of reduced glutathione — via a sulfhydryl group — to electrophilic centers on a wide variety of substrates in order to make the compounds more soluble.This activity detoxifies endogenous compounds such as peroxidised lipids, as well as breakdown of xenobiotics.GSTs may also bind toxins and function as transport proteins,which gave rise to the early term for GSTs of “ligandin”.The mammalian GST super-family consists of cytosolic dimeric isoenzymes of 45–55 kDa size that have been assigned to at least six classes:Alpha,Mu,Pi,Theta,Zeta and Omega.Most mammalian isoenzymes have affinity for the substrate 1-chloro-2,4-dinitrobenzene (CDNB),and spectrophotometric assays utilising this substrate are commonly used to report GST activity.However,some endogenous compounds,e.g., bilirubin,can inhibit the activity of GSTs.In mammals,GST isoforms have cell specific distributions (e.g., alpha GST in hepatocytes and pi GST in the biliary tract of the human liver).

References:

appl_detail:

Western blotting: 1:10000

Datasheet PDF