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Cell/Tissue DNA Extraction Kit

Description

  Cell/Tissue DNA Extraction Kit(Spin-column)
     Cat.#
              Product
          Preps
          Price
   DP 1901
Cell/Tissue DNA Extraction Kit(Spin-column)
            50
          $ 115
   DP 1902
           100
          $ 225
Description
This kit provides a reliable and high-throughput method for the purification of genomic DNA from cell/tissue
 
Primary principle
Cell/tissue material is first mechanically disrupted and then lysed by addition of binding solution/protease K. After lysis, proteins and polysaccharides are salt precipitated. Then the cell debris and precipitates are removed by centrifugation. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the unique silica membrane. The sample is then applied to a spin column and centrifuged. DNA binds to the membrane while contaminants such as proteins and polysaccharides are efficiently removed by two wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
 
Feature and benefits
Rapid, DNA isolation under 30 min
Stable, high-quality silica membrane and ideal buffer system ensure the reproducible results
High purity, Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9
Isolated DNA ranges from 30Kb to 50Kb and can be directly used for most downstream applications, including PCR, Southern-blot, Restriction digestion reactions, etc.
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