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Whole Blood DNA Extraction Kit

Description

  Whole Blood DNA Extraction Kit  (Spin-column)

    Cat.#
           Product
           Preps
          Price
   DP 1801
Whole Blood  DNA extraction kit (spin-column)
             50
          $ 195
   DP 1802
            100
          $ 395
Description
Whole Blood DNA extraction kit is a high-throughout blood DNA isolation system; extracted DNA has high reliability and purity.

Primary principle
In this procedure, whole blood material is first mechanically disrupted and then lysed by addition of binding solution/protease K. After lysis, proteins and polysaccharides are salt precipitated. Then the cell debris and precipitates are removed by centrifugation. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the unique silica membrane. The sample is then applied to a spin column and centrifuged. DNA binds to the membrane while contaminants such as proteins and polysaccharides are efficiently removed by two wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
 
Feature and benefits
Rapid, DNA isolation under 20 min
Stable, high-quality silica membrane and ideal buffer system ensure the reproducible results
Isolated DNA can be directly used for most downstream application, including PCR, Southern-blot, Restriction digestion reactions, etc.
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