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Home > M13 Phage Single Strand DNA Rapid Extraction kit

M13 Phage Single Strand DNA Rapid Extraction kit

Description

 
M13 Phage Single Strand DNA Rapid Extraction kit  (Spin - column)
     Cat. #
         Product
         Preps
          Price
   DP2301
 M13 Phage Single Strand  DNA Rapid Extraction kit (Spin-column)
           50
           $ 85
   DP2302
          100
            N/A
  Description
This kit provides a rapid, reliable and high-throughput method for the purification of high-quality genomic DNA from M13 Phage
 
Primary principle
The infected bacterial culture is centrifuged to pellet the bacterial cell, and then high-salt buffer is added to the supernatant. Single-strand phage DNA is selectively absorbed to silica membrane. The sample is then applied to a spin column and centrifuged. DNA binds to the membrane while contaminants, such as proteins and polysaccharides, are efficiently removed by two washing steps. Pure single-strand phage DNA is eluted in a small volume of low-salt buffer or DNase-free water.
 
Feature and benefits
High yield, generally purify up to 3μg of single-stranded DNA from 800mL of phage supernatant.
Rapid, single sample DNA isolation under 10 min
High purity, Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9 which is suitable for sequencing and other biological applications.
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