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Home > Genomic DNA and RNA Separation Extraction Kit

Genomic DNA and RNA Separation Extraction Kit

Description

gDNA/RNA Separate Extraction Kit ( Spin - column )

    Cat.#    Preps     Prices
  RP 5501      50     $ 250
  RP 5502     100      N/A


Principle
Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine - thiocyanate  - containing buffer, which immediately inactivates RNases to ensure intact RNA. Ethanol is added to provide appropriate binding conditions, and mixture is then applied to the first spin-column, where genomic DNA binds to the membrane, and then adds filtrate to the second spin-column, and total RNA binds to this membrane. Finally, contaminants are efficiently washed away separately. High-quality gDNA and total RNA are then eluted in 30-100 μl water from separate spin-column.
Features
*Stability, comparable RNA yield with high quality absorbing membrane.
*
High-purity, specifically membrane absorption and washing for removing protein and other debris.
*No protein contamination.
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