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Home > Mouse Histidine-rich Glycoprotein(HRG) ELISA Kit

Mouse Histidine-rich Glycoprotein(HRG) ELISA Kit

Description

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HPRG. Standards or samples are then added to the appropriate microtiter plate Wells and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HPRG,enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HPRG in the samples is then determined by comparing the O.D. of the samples to the standard curve
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