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Home > Power HotStart Taq DNA Polymerase

Power HotStart Taq DNA Polymerase

Description

Power HotStart Taq DNA Polymerase
      Cat.#
Preps
Price
    PR 1601
200U
$ 99
    PR 1602
1000U
$ 420

Description
Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is recovery during short 4-minute incubation at 95 ℃. The activated enzyme possesses the same functionality as Taq DNA polymerase: catalyzes 5'→3' synthesis of DNA, having no detectable 3'→5' proofreading exonuclease activity, but possesses low 5'→3' exonuclease activity. It exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Before activation, the two activities are not detectable.
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