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Home > Plasmid Maxi-Preparation Kit

Plasmid Maxi-Preparation Kit

Description

Plasmid Maxi-Preparation Kit (Spin-column)

   Cat.#
           Product
         Preps
         Price
  DP 2501
Plasmid Maxi-Preparation Kit (Spin-column)
            5
          N/A
  DP 2502
           10
        $ 155
Description
This kit can be applied to prepare plasmid  up to 2 mg per column. The purified plasmid is suitable for standard molecular biology applications, such as transfection,  PCR, enzyme digestion, and sequencing.

 
Primary principle
This Kit is depended on an innovative technology applying a rapid nonorganic method of isolating sequencing grade plasmid DNA from E. coli bacterial cultures. It uses modified SDS- alkaline lysis for cell lysis, after lysing the cells, plasmid DNA is selectively captured on silica membrane in the presence of high-salt and low pH value. The bound DNA is then washed with Protein-removing liquid and washing solution, contaminants such as proteins and bacterial debris are efficiently removed. Finally, purified plasmid DNA is eluted in a low-salt buffer.
 
Feature and benefits
High yield, extract up to 2mg DNA at a time
High purity, Purified DNA typically has an A260/A280 ratio between 1.8 and 1.85 and is suitable for sequencing
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